LEAFYX Imaging

LeafYX · Configuration

Instruments, licence and archive, according to the laboratory.

LeafYX controls the acquisition system, is activated by modules with a hardware key and keeps cases in a local or network database. This page summarises what is configured and where.

Supported instruments
LeafYX · Acquisition workstation
0101 0202 0303 0404 0505 0606 0707 0808
  1. 01
    Hardware keyModular licence
  2. 02
    Motorised microscopeModule · Microscope control
  3. 03
    Motorised stageModule · Metaphase Finder
  4. 04
    FocusAutofocus
  5. 05
    Filter wheelModule · Filter wheel control
  6. 06
    CameraModule · Acquisition
  7. 07
    LED illuminationFluorescence, configurable wavelengths
  8. 08
    Oil dispenserModule · Oil dispenser
Illustrative animation: not a screenshot of the program.

01 Supported instruments

Drive your laboratory’s acquisition system.

LeafYX controls the microscope, stage, focus, filter wheel and camera. The devices are chosen and connected from the program options.

Instrument categories

Categories
  • 01
    Motorised microscopesControl of objectives, nosepiece and illumination.
  • 02
    Motorised stages and joystickFor slide scanning and repositioning on the metaphases.
  • 03
    Motorised focusFor autofocus and metaphase finding.
  • 04
    Motorised filter wheelsFor acquisition per fluorochrome.
  • 05
    Digital scientific camerasSelectable in the program options.
  • 06
    LED illuminationFor fluorescence, at configurable wavelengths.
  • 07
    Automatic oil dispenserFor the immersion objective.
  • 08
    Configurable serial connectionsCOM port and baud rate for wheel, microscope, stage and focus.

Compatibility

Check

Compatibility with individual models is checked against your laboratory’s configuration.

  • Tell us which microscope, stage, focus, filter wheel and camera are installed.
  • Together we check which LeafYX functions can be used on your configuration.
  • The control modules (microscope, filter wheel, Metaphase Finder, oil dispenser) are enabled accordingly.

The check is made case by case, on the instruments actually installed.

LeafYX Options window, Acquisition page: main camera, filter wheel, motorised microscope, stage and focus, illumination and thresholds.
FIG. 01Program options, Acquisition page: camera, filter wheel, motorised microscope, stage and focus, with the connection ports.

02 Modular licence

One hardware key, the modules you need.

LeafYX is activated with a hardware key. The archive is always included; the other modules are enabled according to the needs of the laboratory.

ModuleWhat it enablesStatus
ArchiveThe program itself and the case archiveIncluded
AcquisitionLive image, normal and high resolutionModule
KaryotypeManual and automatic karyotypingModule
FISHFISH environmentModule
Filter wheel controlMotorised filter wheelModule
Microscope controlMotorised microscopeModule
Metaphase FinderScanning and automatic metaphase findingModule
Oil dispenserAutomatic oil for the immersion objectiveModule

The modules to enable are defined together with the laboratory, according to its instruments and workflow.

  • Modules8 · the archive is included, the other seven are enabled with the key
  • Languages4 · Italian, English, French, German; others on request
  • Barcodes4 · Code128, Code39, Codebar, Interleaved 2 of 5
  • Import4 formats · TIFF, JPEG, BMP, TGA

03 Archive installation

On the workstation or on the network, with several archives.

Cases are stored in a Microsoft SQL Server Express database. Where to install it, where to keep the images and how to exchange material is decided in the options.

Database

Local or network
  • On the workstationMicrosoft SQL Server Express database.
  • On a shared serverOn the network between several workstations: server location in the options.
  • Several archivesOn the same workstation: create a new archive or load an existing one.

Images

Drives
  • Also on another driveThe image folders can be on a different disk from the database.
  • Service configurationWe set it up with the laboratory.

Archiving

Removable
  • Removable drivesArchiving of cases and restore.
  • Archived cases remain availableThey stay visible as remote cases.
  • CompressionOf archived cases, to take up less space.

Exchange

.cell files
  • Export and importOf cases, single slides and single images, to exchange material between workstations.
  • Rename and deleteOf case, slides and images; deletion asks for confirmation and is recorded in the log.

Case record and barcodes

  • Configurable case recordDefined with a template; import, export and print from the File menu.
  • Format and displayThe barcode format and its display are chosen in the options.
  • AssociationThe Metaphase Finder associates the digits of the barcode with case, slide and scan profile.
Sheet Archive
Database
Microsoft SQL Server Express
Location
Workstation or shared network server
Structure
Case → slides → images
Exchange
Cases, slides and images (.cell)
Barcodes
Code128, Code39, Codebar, Interleaved 2 of 5

04 Users and security

Distinct permissions. Every operation logged: who, what, when.

The audit log is a text file per day and per workstation: one line per event, with date, time, user, case and action.

  • Users with distinct permissions: read, write, administration, backup, export and import; user management from the File menu and sign-in at start-up.
  • Locking of the open case: in multi-user work, a case in use at one workstation is locked for the others.
  • Protected folder: operators can only add lines; reading and deleting is reserved to the administrator.
  • The log is always written on the workstation, even when the case is on the network or on a removable drive.
  • Sign-in and sign-out
  • Case opening and editing
  • Deletion of cases, images and image sets
  • Working windows
  • Metaphase finding
  • Lists, datasets and models
User management window: list of users, password change and administration, read, write, backup, export and import permissions.
FIG. 02User management: passwords and administrator, read, write, backup, export and import permissions.
DateTimeUserAction
User sign-in
Case opened
Case edited
Image deletion
Processing window opened
Metaphase finding started
Model generated · chromosomes · accuracy
Sign-out
Illustrative animation: not a screenshot of the program.1 file per day and workstation

05 Interface and customisation

The interface adapts to those who use it.

The options are organised in pages. The interface language follows that of the operating system; shortcuts and macros speed up repetitive work.

  • Interface in Italian, English, French and German, chosen automatically from the language of the operating system. Other languages can be added on request.
  • Customisable keyboard commands: every button can have its own shortcut.
  • Macros to record and replay the most frequent command sequences.
  • Window layout that can be saved per user, with restoring of the original.
Sheet Interface
Languages
Italian, English, French, German; others on request
Language selection
Automatic, from the language of the operating system
Shortcuts
Customisable, one per button
Macros
Recording and replaying of command sequences

Options pages

  • General
  • Acquisition
  • Display
  • Customisation
  • Metaphase Finder
  • Settings

06 Frequently asked questions

Questions about configuration.

01Does LeafYX work with our instruments?

LeafYX controls motorised microscopes, motorised stages and focus, filter wheels, digital scientific cameras, LED illumination and an oil dispenser, with configurable serial connections. Compatibility with individual models is checked against your laboratory’s configuration: tell us which instruments are installed.

02Can the database be on a network server?

Yes. The Microsoft SQL Server Express database can be on the workstation or on a server shared across the network between several workstations. The server location is set in the options.

03How are the modules activated?

With a hardware key. The archive is always included; acquisition, karyotype, FISH, filter wheel control, microscope control, Metaphase Finder and oil dispenser are enabled according to the needs of the laboratory.

04Which languages is the interface in?

Italian, English, French and German: the program opens in the language of the operating system. Other languages can be added on request.

07 Continue

Learn more.

Classifier

A classifier that learns from your laboratory’s karyotypes.

Training lists, datasets and models per Q, G and R banding, built on the workstation.

Explore the classifier Features

All features, environment by environment.

From the case archive to the report: LeafYX commands, options and tools.

See the features

For Research Use Only.

LeafYX is software for cytogenetics research. For Research Use Only (RUO): not a medical device and not for use in diagnostic procedures.

The automatic functions produce suggestions: separation, classification, karyotype and count remain under the operator’s verification.

Demo

Check LeafYX on your own configuration.

Contact us for a demonstration, to check the installed instruments or to define the modules best suited to your laboratory.

Request a demo Explore the classifier