LEAFYX Imaging

LeafYX · Overview

LeafYX Six environments, one case at the centre.

A software workstation for cytogenetics research: it drives the microscope, camera and motorised stage, archives preparations by case, slide and image, and guides the operator through to the karyotype, the count and the visual analysis.

Explore the features
LeafYX · Main Case › Slide › Image

01 / 06 · Main

Archive, case structure, gallery.

Illustrative animation: not a screenshot of the program.

Environment features

01 Benefits for the lab

Fewer separate steps, more control over the result.

A single program takes the preparation from acquisition to the report, and the choices that matter stay with the operator.

B.01 One case

Linked data, images and results.

Gallery, metaphases, karyotypes, fusions and FISH images stay in the same archive, linked to case, slide and image.

B.02 Less repetitive work

Less time at the microscope.

The motorised stage scans the slide and returns by itself to the metaphases chosen for high-magnification acquisition.

B.03 Operator review

Suggestions never replace the expert.

Separation, classification and karyotype layout are assisted; every chromosome can still be corrected and reclassified.

B.04 Traceability

Every operation logged: who, what, when.

The audit log writes one line per event, with date, time, user, case and action, always on the workstation.

02 Environments

Six environments, each with its own commands.

The main window stays the same: the environment is chosen from a side bar and a top bar. Each card leads to the features in detail.

01 Main

Archive, case structure, gallery.

Case archive, case structure with slides and images, gallery with thumbnails by type: metaphase, karyotype, fusion, composition.

 02 Acquisition

Live or with automatic finding.

Live camera image, control of the microscope and stage, autofocus and automatic metaphase finding.

 03 Processing

Separation, karyotype and ideograms.

Metaphase, karyotype and composition in three areas; chromosome separation, manual and automatic karyotyping, ideograms.

 04 Fusion

Several captures of the same field.

Chromosomes left outside the image are transferred from the capture to the metaphase, tagged with the label of the fusion they come from.

 05 Count and visual analysis

Count and check the classes.

One click per chromosome; the class buttons change appearance according to how many chromosomes they have received.

 06 FISH

FISH.

Processing of FISH images, with configurable fluorochromes and probes.
Screenshot of the main LeafYX window in the Processing environment: karyotype, metaphase with the fusion captures and the image gallery of a sample case.
FIG. 01LeafYX window, Processing environment: karyotype, metaphase with the chromosomes recovered from captures F1 and F2, case gallery.

All features, environment by environment

03 FISH and fluorescence

One channel per fluorochrome, one recombined image.

In fluorescence and FISH, acquisition is per fluorochrome, one channel per filter. The channels are then recombined into a single colour image.

  1. 01
    Acquisition per fluorochrome

    With a motorised filter wheel and, where fitted, LED illumination at configurable wavelengths.

  2. 02
    Colour recombination

    Names and colours of fluorochromes and probes are configurable; the counterstain is selectable.

  3. 03
    Measurements on FISH preparations

    Measurement of optical density and evaluation of the signal ratio.

The FISH environment in detail

  • DAPI counterstain
  • FITC probe
  • Cy3 probe
  • Cy5 probe
FIG. 02Illustrative animation: not a screenshot of the program. Fluorochrome names and colours are examples: they can be configured in LeafYX.

04 Trainable classifier

A classifier that learns from your laboratory’s karyotypes.

Automatic karyotyping uses a classifier chosen in the options. For each banding type, Q, G and R, the laboratory can use its own model, built on the workstation from the karyotypes it has verified.

  • Training lists with one click from Processing.
  • Accuracy measured at each model generation.
  • Automatic model selection based on the case identifier.
  • Dataset and model are built locally: the images stay on the workstation.

Explore the classifier

05 Configuration and modules

Instruments and modules, tailored to the laboratory.

LeafYX drives the motorised microscope, stage, focus, filter wheels and digital camera. The hardware key enables the modules your workflow needs.

  • Archive on Microsoft SQL Server Express, on the workstation or on a shared network server.
  • Users with distinct permissions; a case in use is locked for the other workstations.
  • The hardware configuration is checked together, before installation.

See the configuration

Hardware key Modules that can be enabled
  • Archivealways included
  • Acquisitionlive image
  • Karyotypemanual and automatic
  • FISHFISH environment
  • Filter wheelmotorised control
  • Microscopemotorised control
  • Metaphase Finderslide scanning
  • Oil dispenserimmersion oil
Supported instruments
MicroscopeStageFocusFilter wheelCameraLED illumination

06 LeafYX in figures

24classes

Classes of the human karyotype: 1–22, X and Y.

3banding types

Q, G and R, each with its own trainable model.

400 · 550 · 850

Ideogram bands, including R bands.

4languages

Italian, English, French and German. Other languages on request.

4formats

TIFF, JPEG, BMP and TGA for import.

07 Frequently asked questions

Frequently asked questions.

More questions? Write to us: we also answer questions about setting up the workstation.

01What does “for research use only” mean?

LeafYX is software for cytogenetics research. For Research Use Only (RUO): not a medical device and not for use in diagnostic procedures. The automatic functions produce suggestions that the operator verifies.

02Which images can be acquired or imported?

You can acquire in brightfield, fluorescence and FISH; in fluorescence and FISH, one channel per fluorochrome. You can also import images from disk in TIFF, JPEG, BMP and TGA formats.

03Can the automatic karyotype be corrected?

Yes. Each chromosome can be moved, swapped, rotated and flipped by hand, and reclassified.

04Where are images, datasets and models kept?

Cases are stored in a Microsoft SQL Server Express database, on the workstation or on a shared network server. The classifier’s datasets and models are built locally: the images stay on the workstation.

05Which languages is the interface available in?

Italian, English, French and German: the program opens in the language of the operating system. Other languages can be added on request.

06Is a new version planned?

The new generation of LeafYX is in development, with a renewed interface and full compatibility with existing archives.

For Research Use Only.

LeafYX is software for cytogenetics research. For Research Use Only (RUO): not a medical device and not for use in diagnostic procedures.

The automatic functions produce suggestions: separation, classification, karyotype and count remain under the operator’s verification.

Illustrative animations: not screenshots of the program.

Demo

Evaluate LeafYX on your own laboratory’s workflow.

Contact us for a demonstration, to check your hardware configuration or to define the modules best suited to your lab.

Request a demo Explore the features